In this tutorial, we will use "indexed file formats", which exemplifies using plain text configuration
Loading Indexed FASTA
To begin, we'll pretend as though we are setting up the genome of Volvox mythicus, a mythical species in the genus Volvox. The Volvox genome was sequenced by your sequencing core in 2018 and they'd like to setup JBrowse now. They give us a link to their FASTA file that we'll download
mkdir data curl -L https://jbrowse.org/code/JBrowse-1.16.5/docs/tutorial/data_files/volvox.fa > data/volvox.fa
We are going to use samtools to create a "FASTA index" using their faidx command. FASTA indexing allows even very large FASTA files to be downloaded into JBrowse "on demand" e.g. only downloading the sequence required for a certain view.
samtools faidx data/volvox.fa
The FASTA index will be a file called volvox.fa.fai. Then we'll move these files into a "data directory" that JBrowse can use
Then create the file data/tracks.conf with this file content
[GENERAL] refSeqs=volvox.fa.fai [tracks.refseq] urlTemplate=volvox.fa storeClass=JBrowse/Store/SeqFeature/IndexedFasta type=Sequence
Now your directory structure is something like
/var/www/jbrowse /var/www/jbrowse/data /var/www/jbrowse/data/tracks.conf /var/www/jbrowse/data/volvox.fa /var/www/jbrowse/data/volvox.fa.fai
At this point, you should be able to open up http://localhost/jbrowse/?data=data (or just simply http://localhost/jbrowse/) and you will see your genome with the reference sequence track. If you have any problems at this stage, send an email to firstname.lastname@example.org with details about your setup for troubleshooting, or file a GitHub issue.
Loading Tabix GFF3
We will use the newly generated "gene annotation" file that was generated for Volvox mythicus
curl -L https://jbrowse.org/code/JBrowse-1.16.5/docs/tutorial/data_files/volvox.gff3 > data/volvox.gff3
When we are processing GFF3 for usage in JBrowse, we can aim to use GFF3Tabix format. Tabix allows random access to genomic regions similar to Indexed FASTA. We must first sort the GFF to prepare it for tabx
sort -k1,1 -k4,4n data/volvox.gff3 > data/volvox.sorted.gff3 (grep ^"#" data/volvox.gff3; grep -v ^"#" data/volvox.gff3 | grep -v "^$" | grep "\t" | sort -k1,1 -k4,4n) > data/volvox.sorted.gff3
This command extracts the header then sorts the GFF without the header. You can also use GenomeTools with
gt gff3 -sortlines data/volvox.gff3 > data/volvox.sorted.gff3 if that is easier. Then run
bgzip data/volvox.sorted.gff3 tabix -p gff data/volvox.sorted.gff3.gz
Then we can create a gene track in data/tracks.conf
[tracks.genes] urlTemplate=volvox.sorted.gff3.gz storeClass=JBrowse/Store/SeqFeature/GFF3Tabix type=CanvasFeatures
If you have been given sequenced alignments, you can also create a Alignments track that displays the alignments
For volvox, we are given a file
curl -L https://jbrowse.org/code/JBrowse-1.16.5/docs/tutorial/data_files/volvox-sorted.bam > data/volvox-sorted.bam
Note that this BAM file is already sorted. If your BAM is not sorted, it must be sorted to use in JBrowse. Next index this file
samtools index data/volvox-sorted.bam
Finally add this content into data/tracks.conf
[tracks.alignments] urlTemplate=volvox-sorted.bam storeClass=JBrowse/Store/SeqFeature/BAM type=Alignments2
Note that as of JBrowse 1.15.0, CRAM format is also supported, simply switch .bam and .bam.bai with .cram and .cram.crai and use JBrowse/Store/SeqFeature/CRAM
Check that your files are "loaded"
At this point, if the jbrowse files are in your webserver, you should have a directory layout such as
/var/www/html/jbrowse /var/www/html/jbrowse/data /var/www/html/jbrowse/data/volvox.fa /var/www/html/jbrowse/data/volvox.fa.fai /var/www/html/jbrowse/data/volvox.sorted.gff3.gz /var/www/html/jbrowse/data/volvox.sorted.gff3.gz.tbi /var/www/html/jbrowse/data/volvox-sorted.bam /var/www/html/jbrowse/data/volvox-sorted.bam.bai /var/www/html/jbrowse/data/tracks.conf
Then your tracks.conf file should say
[GENERAL] refSeqs=volvox.fa.fai [tracks.refseq] urlTemplate=volvox.fa storeClass=JBrowse/Store/SeqFeature/IndexedFasta type=Sequence [tracks.genes] urlTemplate=volvox.sorted.gff3.gz storeClass=JBrowse/Store/SeqFeature/GFF3Tabix type=CanvasFeatures [tracks.alignments] urlTemplate=volvox-sorted.bam storeClass=JBrowse/Store/SeqFeature/BAM type=Alignments2
Then you can visit http://localhost/jbrowse/ and the "data" directory will automatically be loaded.
You have now setup JBrowse!
If you have troubles, send an email to email@example.com or create a GitHub issue (note that GitHub issues tend to be for pure concerns about JBrowse having bugs, so email list is preferre).
a) If the folder was not called data, e.g. you had your files in /var/www/html/jbrowse/otherdata, then you can visit http://localhost/jbrowse/?data=otherdata (this automatically lends a way to have "multiple data directories" since you could navigate to different ?data= URL paths this way. the "dataset selector" configuration contains more details)
b) The "configuration format" is called .conf, but JSON format is also allowed. See configuration file formats guide for details.
c) If you are doing source code updates or using plugins, you must use a git clone or the "source code" release from GitHub pages. Also the "-dev" release from older versions is supplanted by using a git clone or "source code" release