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Quantitative tracks

Visualizing genome stopTokens, whether it is read depth-of-coverage or other stopToken, can often be done by using BigWig or other quantitative feature files.

A simple wiggle track with the XY plot renderer.
Figure: A simple wiggle track with the XY plot renderer.

Viewing whole-genome coverage for profiling CNV

You can refine the resolution of BigWig tracks, and view whole genome coverage to get detailed global views of CNV, for example from whole-genome coverage profiling.

Here is a short picture guide to setup a whole-genome view of a BigWig for CNV coverage visualization:

  1. Open your BigWig track
  2. Go to the view menu and select "Show all assembly regions"
  3. Adjust the "Autoscale type" to your liking, the new options for "Local +/- 3sd" allows the autoscaling to avoid outliers
  4. Go to the track menu and select "Turn off histogram fill", which then shows only a dot for each point on the graph
  5. Go to the track menu and select "Resolution->Finer resolution" a couple times until resolution looks nice
Note

All tracks have a drag handle on the bottom, which you can drag down to make the track taller.

A step-by-step guide to view a whole-genome CNV profile of coverage from a BigWig file.
Figure: A step-by-step guide to view a whole-genome CNV profile of coverage from a BigWig file.